Skip to main content
. 2009 Feb 24;4(2):e4580. doi: 10.1371/journal.pone.0004580

Figure 3. β-catenin is targeted to the perinuclear endocytic recycling compartment.

Figure 3

(A) A431 cells were serum-starved, pretreated with 1 µM LPA for 1 h, and incubated for 30 min in 10 µg/ml Alexa-Fluor™ 647-transferrin (colored as green). Cells were fixed, stained with anti-β-catenin monoclonal IgG antibody (colored as red), and imaged using a Zeiss LSM-510 confocal microscope (40× Plan-NEOFLUAR objective; NA 1.3; scale bar = 50 µm). The perinuclear region stained positively for transferrin under receptor recycling conditions and overlapped well, colocalizing with ß-catenin staining. (B) The distribution of β-catenin at the perinuclear region was also performed with anti-Rab11 polyclonal rabbit antibody. Cells were incubated in PBS or 1 µM LPA for 1.5 h, fixed, stained with anti-β-catenin monoclonal antibody (red) and Rab11 antibody (green), and imaged with a Zeiss LSM-510 confocal microscope. Rab11 staining was also found to highly colocalized with β-catenin staining (yellow; arrows) (N = 3 per group).