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. 2009 Feb 27;5(2):e1000395. doi: 10.1371/journal.pgen.1000395

Figure 4. Reduced expression of Tbx1 in 129S6- and B6-Chrd−/− embryos.

Figure 4

In situ hybridization at 5-somite stage of Tbx1 in 129S6-Chrd+/+ (A,C) and 129S6-Chrd−/− embryos (B,D), displaying reduced expression in pharyngeal area of homozygotes with a DGS-like phenotype. (E) Quantitative PCR of Tbx1 mRNA in embryos of the genotypes B6-Chrd +/+ and B6-Chrd +/− (n = 8) versus B6-Chrd−/− (n = 4), demonstrating a modest but significant (p = 0.029) reduction of Tbx1 expression despite the absence of a DGS-like phenotype in B6-Chrd−/− mutants.