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In vivo association of p53 with Sp1 and NPM but not with p65 was revealed by co-immunoprecipitation (IP) analysis in isolated nuclear extract (panel a). Both p65 and Sp1 were co-immunoprecipitated with p50 antibody, but p53 was not (panel b). Co-immunoprecipitation with normal IgG was shown as control (panel c). DMSO, Me2SO.
