Table 1.
Growth suppression of H1299 cells following transfection with mutant p53 expression plasmids
| Plasmid | No. of drug-resistant
colonies*
|
Average† | |||
|---|---|---|---|---|---|
| Exp 1 | Exp 2 | Exp 3 | Exp 4 | ||
| Vector | 410 (1.00) | 510 (1.00) | 325 (1.00) | 540 (1.00) | |
| Wild-type p53 | 48 (0.12) | 53 (0.10) | 28 (0.09) | 55 (0.10) | 0.10 |
| 22-23 | 250 (0.61) | 334 (0.65) | 195 (0.60) | 273 (0.51) | 0.59 |
| R175H | 425 (1.04) | 525 (1.03) | 390 (1.20) | 510 (0.94) | 1.03 |
| ΔproAE | 310 (0.76) | 260 (0.51) | 180 (0.55) | 253 (0.47) | 0.57 |
The data shown represent the number of drug-resistant colonies counted 2 weeks following transfection with 10 μg of the indicated plasmid.
*
Numbers in parentheses represent the calculated ratio of colony number relative to the number scored on the empty vector control plate.
†
The average of the four ratio values determined for each plasmid is presented in the last column.