Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Jun;32(6):1604–1605. doi: 10.1128/jcm.32.6.1604-1605.1994

Evidence of pestivirus RNA in human virus vaccines.

R Harasawa 1, T Tomiyama 1
PMCID: PMC264050  PMID: 8077414

Abstract

We examined live virus vaccines against measles, mumps, and rubella for the presence of pestivirus RNA or of pestiviruses by reverse transcription PCR. Pestivirus RNA was detected in two measles-mumps-rubella combined vaccines and in two monovalent vaccines against mumps and rubella. Nucleotide sequence analysis of the PCR products indicated that a modified live vaccine strain used for immunization of cattle against bovine viral diarrhea is not responsible for the contamination of the vaccines.

Full text

PDF
1605

Images in this article

1604Image
on p.1604

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchio A. F. Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology. 1988 Jul;165(1):191–199. doi: 10.1016/0042-6822(88)90672-1. [DOI] [PubMed] [Google Scholar]
  2. Deng R., Brock K. V. Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1. Virology. 1992 Dec;191(2):867–869. doi: 10.1016/0042-6822(92)90262-n. [DOI] [PubMed] [Google Scholar]
  3. Giangaspero M., Wellemans G., Vanopdenbosch E., Belloli A., Verhulst A. Bovine viral diarrhoea. Lancet. 1988 Jul 9;2(8602):110–110. doi: 10.1016/s0140-6736(88)90047-5. [DOI] [PubMed] [Google Scholar]
  4. Kreeft H. A., Greiser-Wilke I., Moennig V., Horzinek M. C. Attempts to characterize bovine viral diarrhea virus isolated from cattle after immunization with a contaminated vaccine. Dtsch Tierarztl Wochenschr. 1990 Feb;97(2):63–65. [PubMed] [Google Scholar]
  5. Løken T., Krogsrud J., Bjerkås I. Outbreaks of border disease in goats induced by a pestivirus-contaminated orf vaccine, with virus transmission to sheep and cattle. J Comp Pathol. 1991 Feb;104(2):195–209. doi: 10.1016/s0021-9975(08)80103-x. [DOI] [PubMed] [Google Scholar]
  6. Meyers G., Rümenapf T., Thiel H. J. Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology. 1989 Aug;171(2):555–567. doi: 10.1016/0042-6822(89)90625-9. [DOI] [PubMed] [Google Scholar]
  7. Meyers G., Rümenapf T., Thiel H. J. Ubiquitin in a togavirus. Nature. 1989 Oct 12;341(6242):491–491. doi: 10.1038/341491a0. [DOI] [PubMed] [Google Scholar]
  8. Nuttall P. A., Luther P. D., Stott E. J. Viral contamination of bovine foetal serum and cell cultures. Nature. 1977 Apr 28;266(5605):835–837. doi: 10.1038/266835a0. [DOI] [PubMed] [Google Scholar]
  9. Potts B. J., Sever J. L., Tzan N. R., Huddleston D., Elder G. A. Possible role of pestiviruses in microcephaly. Lancet. 1987 Apr 25;1(8539):972–973. doi: 10.1016/s0140-6736(87)90311-4. [DOI] [PubMed] [Google Scholar]
  10. Qi F., Ridpath J. F., Lewis T., Bolin S. R., Berry E. S. Analysis of the bovine viral diarrhea virus genome for possible cellular insertions. Virology. 1992 Jul;189(1):285–292. doi: 10.1016/0042-6822(92)90704-s. [DOI] [PubMed] [Google Scholar]
  11. Robertson J. S. New molecular approaches for detecting viral genomes. Dev Biol Stand. 1991;75:205–209. [PubMed] [Google Scholar]
  12. Roehe P. M., Woodward M. J., Edwards S. Characterisation of p20 gene sequences from a border disease-like pestivirus isolated from pigs. Vet Microbiol. 1992 Nov;33(1-4):231–238. doi: 10.1016/0378-1135(92)90051-t. [DOI] [PubMed] [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vannier P., Leforban Y., Carnero R., Cariolet R. Contamination of a live virus vaccine against pseudorabies (Aujeszky's disease) by an ovine pestivirus pathogen for the pig. Ann Rech Vet. 1988;19(4):283–290. [PubMed] [Google Scholar]
  15. Wensvoort G., Terpstra C. Bovine viral diarrhoea virus infections in piglets born to sows vaccinated against swine fever with contaminated vaccine. Res Vet Sci. 1988 Sep;45(2):143–148. [PubMed] [Google Scholar]
  16. Wilks C. R., Abraham G., Blackmore D. K. Bovine pestivirus and human infection. Lancet. 1989 Jan 14;1(8629):107–107. doi: 10.1016/s0140-6736(89)91469-4. [DOI] [PubMed] [Google Scholar]
  17. Yolken R., Dubovi E., Leister F., Reid R., Almeido-Hill J., Santosham M. Infantile gastroenteritis associated with excretion of pestivirus antigens. Lancet. 1989 Mar 11;1(8637):517–520. doi: 10.1016/s0140-6736(89)90066-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES