Table 1.
Pretransplant transduction efficiency
| Patient no. | Transduction sample no. | Cancer type | In situ PCR | Plating efficiency | Solution PCR
|
|
|---|---|---|---|---|---|---|
| Without nesting | With nesting | |||||
| Suspension | Suspension | Suspension | Suspension | |||
| O1 | 1 | Ovary | ND | 2.0 | + | + |
| O2 | 2 | Ovary | ND | 8.4 | NE | NE |
| O3 | 3 | Ovary | ND | 5.7 | − | + |
| O4 | 4 | Ovary | ND | 16.5 | ND | + |
| O5 | 5 | Ovary | 3.0 | 5.7 | ND | + |
| O6 | 6 | Ovary | 1.2 | 20.1 | − | + |
| O9 | 7 | Ovary | 1.9 | 6.0 | + | ND |
| B1 | 8 | Breast | ND | 2.5 | ND | + |
| B2 | 9 | Breast | ND | 18.1 | − | + |
| B3 | 10 | Breast | 5.3 | 2.3 | − | + |
| Mean | 2.8 | 8.7 | ||||
| Median | 1.9 | 5.7 | ||||
| Stromal | Stromal | Stromal | Stromal | |||
| O7 | 1 | Ovary | 3.6 | 8.1 | + | ND |
| O8 | 2 | Ovary | 1.8 | 9.4 | + | ND |
| O10 | 3 | Ovary | 8.1 | 2.1 | + | ND |
| B4 | 4 | Breast | 6.9 | −0.22 | + | ND |
| B5 | 5 | Breast | 9.0 | 14.7 | + | ND |
| B6 | 6 | Breast | 3.0 | 4.3 | + | ND |
| B7 | 7 | Breast | 2.5 | 2.2 | + | ND |
| B8 | 8 | Breast | 8.9 | 2.7 | + | ND |
| B9 | 9 | Breast | 3.7 | 1.0 | + | ND |
| B10 | 10 | Breast | 6.1 | 1.0 | + | ND |
| Mean | 5.4 | 4.5 | ||||
| Median | 6.1 | 2.7 | ||||
The method of transduction (suspension or stromal) is indicated above each column of data. ND, not done; NE, not evaluable.