Table 1.

Primers and restriction enzymes for polymorphisms

Gene	Primer sequences (5′-3′)	Product size, kb	Restriction enzyme	GenBank name (ref.)
Cola1	AGCCCGGTAGTAGCGGCCAC	2.0	AluI	HUMCG1PA1
	GCAAGTGGTGAACGTGGTCC
Itg3a	GGGGCTCATCATCCTCCTCTTGTG	0.4	none	HUMINTA3A (24)
	CTTCATCTCCGCCTTCTGCCTCTT			S66292 (25)
Ngfr	GTTCTCCTGCCAGGACAAACAGAACAC	0.2	RsaI	RNNGFRR (26)
	GCATTCAGCATCAGCCCAGGGCGTGCA
Ngfr	AGCCGCCCTGCGACGCATCCA	1.4	HinfI	RNNGFRR (26)
	GCCATGAAACTGAGGAGAAAGTTG			∗
Gip	AGAGAGAGGCCCGGGCTTTGGA	0.9	HaeIII	MMU34295
	CCAGGCCAGTAGCTCTTGAATCAG			HUMGIPX5 (27)
Hoxb9	GCATGAAGTGGCCAGACTCCTCAAT	1.2	AluI	MUSHOX25A (28)
	CAATGGGAGACTTGTCTTTGCTGGT		RsaI
Hoxb1	CTGGATGAAGGTCAAGAGAAACCCA	1.3	DdeI	MMHOX29 (29)
	TATGTCACAAGGCAGTCGGTGCTAT		HphI
Cox5a	CAGGCAACTATTTCAATCAAGTAGC	0.5	DdeI	MMCOXVA (30)
	GTTCCATTCGGTGCTATTCTCATG
Itgb3	TCCAGCTCATTGTTGATGCTTATGG	1.1	RsaI	HUMGP3A (31)
	GGTCACGCACTTCCAGCTCGACTTT

Restriction enzymes listed detect polymorphic sites between RIIIS/J and CASA/Rk mice. When two enzymes are listed, both were polymorphic between strains. Product sizes are approximate. Itg3a is a length polymorphism; the RIIIS/J product is ≈50 bp larger than the CASA/Rk product. When two GenBank references are listed, both were used to design primers. ∗, Based on direct sequence analysis of murine PCR product (see text). ref., reference number.