Fig. 5. Colocalization of CHT with VAChT and of TH with VMAT2 in atrial nerve fibers.

(A–C) The cholinergic markers CHT (A) and VAChT (B) were localized to identical sites in nerve fibers, resulting in a yellow color at the points of colocalization in the overlay image (C). (D–F) Similarly, the noradrenergic markers TH (D) and VMAT2 (E) were also colocalized in nerve fibers (F). Panels (A–F) show maximum projection images compiled from confocal scans that spanned 8 µm, and inserts show single optical sections from the boxed regions at higher magnification. Scale bars indicate 100 µm in (A–C) and (D–F).