Fig. 3.

Kinetics of aptamer refolding and binding. The fraction of FECs corresponding to the fully-folded, adenine-bound aptamer (identified by the appropriate unfolding signature) for various adenine concentrations as a function of the variable time delay for refolding between pulls. Solid curves display the global fit to a minimal 3-state kinetic scheme (inset): “U” = unfolded, “A-comp” = competent to bind adenine, “F” = folded; adenine-bound.