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. Author manuscript; available in PMC: 2009 Dec 20.
Published in final edited form as: Virology. 2008 Oct 31;382(2):257–266. doi: 10.1016/j.virol.2008.09.018

Fig. 4.

Fig. 4

Cellular miRNA primary transcripts differentially regulated during EBV latency. Transcript-specific PCR primers were designed to amplify the primary transcript of cellular miRNAs differentially regulated during EBV latency. Total RNA from the following immortalized B cell lines was isolated and transcribed into cDNA: a. EBV-positive, latency III cell lines Jijoye, JY, IB4, and X50-7; b. EBV-positive, latency I cell lines Akata, Rael and Mutu; c. EBV negative Burkitt’s lymphoma cell lines DG75 and Ramos. The cDNAs were tested for mature cellular miRNA species by real-time, quantitative PCR in triplicate, and results were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. A–D. pri-miR-21, pri-miR-28 (LPP transcript), pri-miR-34a and pri-miR-146b, respectively.