Fig. 3.

Ai: typical morphology of bladder interstitial cell. Aii–iii: time series fluorescence micrograph showing Ca²⁺ response to 1 μM carbachol which was blocked by the M₃ antagonist 4-DAMP (1 μM). Traces show intensity time series of responses to carbachol and the effects of 4-DAMP and the M₂ antagonist methoctramine (1 μM). Fluorescence (F) of an event is expressed as a ratio of background (F₀). CCh, carbachol. B: graph summarizing the effect of 4-DAMP and methoctramine on 7 and 11 cells, respectively. *Statistical significance.