FIGURE 2.

mAb-112 inhibits in vitro plasminogen activation initiated by Pro-uPA or uPA. 0.25 nm pro-uPA (panel A) or 0.25 nm uPA (panel B) was incubated without (black dots) or with 5 nm (red dots), 10 nm (green dots), 20 nm (yellow dots), 40 nm (dark blue dots), 80 nm (pink dots), and 160 nm (light blue dots) of mAb-112. After incubation for 30 min at room temperature, plasminogen was added to 0.5 μm and S-2251 to 0.5 mm. Substrate hydrolysis was followed at 37 °C by measuring the absorbance at 405 nm. Pro-uPA or uPA was omitted in controls (gray curves).