FIGURE 5.

mAb-112 hinders the cleavage event in plasmin-mediated pro-uPA activation. Aliquots of pro-uPA (200 nm) were incubated with plasmin (5 nm, panel A) or matriptase (50 nm, panel B) in the absence or presence of mAb-112 (300 nm). After the indicated incubation periods, the reaction products were analyzed by reducing SDS-PAGE and immunoblotting with a polyclonal anti-uPA antibody. The cleavage was observed as the conversion of the M_r ∼ 54,000 band of single-chain pro-uPA to the M_r ∼ 30,000 catalytic domainand M_r ∼ 20,000 amino-terminal fragment. tc, two chain.