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. 2009 Feb 13;284(7):4373–4382. doi: 10.1074/jbc.M807259200

FIGURE 1.

FIGURE 1.

Bid-/- mice are significantly protected from liver injury induced by ConA/GalN, but not ConA alone. A, wild-type (Bid+/+) and Bid-deficient (Bid-/-) mice were treated with ConA or ConA/GalN for 8 h and sacrificed. The liver sections were stained with hematoxylin and eosin. Significantly less injury were observed in the Bid-deficient livers following ConA/GalN treatment. B, wild-type (Bid+/+) and Bid-deficient (Bid-/-) mice were treated with ConA/GalN for 6 h and sacrificed. The liver sections were stained with phalloidin (red) and Hoechst 33342 (blue). Disruption of the liver parenchymal and apoptotic nuclei could be clearly detected in the wild type mice but not in the Bid-deficient mice at this time. C, liver lysates from wild-type (Bid+/+) and Bid-deficient (Bid-/-) mice treated with ConA for 8 h were analyzed by immunoblot with an anti-caspase-3 antibody. Each line represents one mouse sample. C, nontreated; 1, 2, and 3, three treated samples; P, a positive control sample of wild type mice treated with LPS/GalN. D, liver lysates from wild-type (Bid+/+) and Bid-deficient (Bid-/-) mice treated with ConA/GalN and sacrificed at the designated time point were analyzed by immunoblot with the indicated antibodies. C-8, caspase-8; C-9, caspase-9; C-3, caspase-3.