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Expression of transcription factors in osteoclast differentiation. Infected cells were treated with RANKL (50 ng/ml) for 3 days, and nuclear extracts were analyzed by immunoblotting with antibodies against PU.1 (A), MITF (B), c-Fos (C), and NFATc1 (D). Quantitative image analysis of the protein expression levels (values below bands) was normalized to histone H3. IB, immunoblotting.
