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Expression of osteoclast-specific markers during osteoclastogenesis. Infected cells were treated with RANKL (50 ng/ml) for 3 days. Total RNA was extracted at the indicated time points, and expression of TRAP (A), MMP9 (B), Ctsk (C), CTR (D), and integrin β3 (E) mRNA levels were measured by real time RT-PCR. The PCR products were normalized to GAPDH for each reaction. The data represent the means ± S.D. of three experiments in triplicate.
