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. 2009 Feb 13;284(7):4292–4299. doi: 10.1074/jbc.M807503200

FIGURE 6.

FIGURE 6.

Effects of Dgat1 deficiency on retinoid homeostasis are epidermis-autonomous. a, specific deletion of Dgat1 in epidermis. Shown is PCR detection of the wild-type and floxed allele of Dgat1 and Cre transgene in genomic DNA. Interleukin-2 (IL-2) served as an internal PCR control. The absence of a PCR band indicates Cre-mediated recombination. b, absence of Dgat1 mRNA in the epidermis of K14-Cre+Dgat1flox/flox (EP-D1KO) mice. mRNA levels of Dgat1 in the tail epidermis and WAT were quantified by real time PCR. (age 13 weeks, n = 3–6 mice/group). c, cyclical alopecia in EP-D1KO mice. Alopecia was detectable by 7.5 weeks of age. Note partial hair regrowth by 10.5 weeks of age. Alopecia was not observed in control mice. (n = 6/genotype). d, increased mRNA expression of RA target genes in whole skin of EP-D1KO mice. mRNA levels were quantified by real time PCR (age 13 weeks, n = 5/genotype). *, p < 0.0001; **, p = 0.0002 versus control.