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. 2009 Feb 13;284(7):4473–4483. doi: 10.1074/jbc.M806010200

FIGURE 7.

FIGURE 7.

In vivo attenuation of venous thrombosis by TF Ab. A, specific binding of anti-mTF Ab to human and murine TF. Detergent lysates of stimulated HUVECs and murine macrophages were immunoblotted by the rabbit anti-mTF polyclonal Ab. B and C, inhibition of human and murine TF activity by anti-mTF Ab. HUVECs and murine macrophages were stimulated with LPS, TNF-α, and PMA, and the activity of human and murine TF was determined in the presence of rabbit preimmune IgG and anti-mTF Ab. Results are presented as the mean ± S.D. value of triplicate measurements of three independent experiments (*, p < 0.05; **, p < 0.01 versus rabbit IgG group). D, thrombotic mice were either untreated (n = 7) or treated with Andro (n = 13), anti-mTF Ab (n = 11), Andro plus the anti-mTF (n = 10), or rabbit preimmune IgG (n = 6). After 6 days, venous thrombi were removed, weighed, and measured for length. The weight was then normalized to length. Results are presented as the mean ± S.D. value from two separate experiments (**, p < 0.01 versus model group).