FIGURE 9.

Interaction of transfected and endogenous TRPC3 and TRPC6. A, V5-TRPC3 and/or FLAG-TRPC6 were expressed in HEK 293T cells with Epo-R. Immunoprecipitation (IP) was performed on lysates with anti-V5 antibody or anti-FLAG-agarose. Western blotting (WB) was performed after immunoprecipitation with anti-V5 or anti-FLAG antibodies. Representative results of five experiments are shown. B, immunoprecipitation was performed on lysates from TF-1 erythroid cells with anti-TRPC3 or anti-TRPC6 antibody to detect endogenous interactions. Western blotting was performed after immunoprecipitation with anti-TRPC3 or anti-TRPC3 antibodies. Representative results of two experiments are shown.