FIGURE 5.

Overexpression of mTOR suppresses PKC-δ- and Akt-induced NF-κB activity in endothelial cells. A, HUVEC were transfected with NF-κBLUC in combination with pcDNA3-PKCδ-CAT encoding constitutively active PKC-δ (PKC-δ-CAT) and pRK5-mTOR-WT encoding wild type mTOR (mTOR-WT) using DEAE-dextran as described under “Experimental Procedures.” B, HUVEC were transfected with NF-κB LUC in combination with pcDNA3-Akt-CAT encoding constitutively active Akt (Akt-CAT) and pRK5-mTOR-WT using Lipofectamine 2000 as described under “Experimental Procedures.” After 18 h, cell extracts were prepared and assayed for firefly and Renilla luciferase activities. Data are mean ± S.E. (n = 6 to 9 for each condition). *, p < 0.05 compared with vector-transfected control; #, p < 0.01 compared with PKCδ-CAT- or Akt-CAT-transfected control.