FIGURE 3.

CspWT overexpression increases the association of CHIP with CFTR. A, HEK293 cells were transfected and immunoprecipitated as in Fig. 2. The immunoprecipitated material was analyzed by SDS-PAGE and immunoblotting with rabbit anti-CHIP (upper panel) or mouse anti-CFTR (lower panel). B, quantitation of the CHIP co-immunoprecipitated with CFTR normalized to the amount of immunoprecipitated CFTR and expressed as the -fold change relative to Sar1-GTP overexpression (n = 3).