TABLE 2.

Stopped-flow kinetics values for different forms of the eEF1A:eEF1Bα complex

eEF1Bα	Maximal koff of GDP	Kd of complex	Source
	s−1	μm
Wild type	122.0 ± 8.0	4.0 ± 0.8	(3)
F163A	4.3 ± 0.3	25.7 ± 6.9	This study
W130A	230.9 ± 20.0	2.6 ± 0.9	This study
W130A/F163A	7.8 ± 0.6	25.5 ± 7.0	This study
K205A	8.0 ± 0.3	0.4 ± 0.1	(3)
F163A/K205A	3.8 ± 0.2	11.3 ± 2.1	This study