TABLE 1.
Replication fork DNA polymerases
The large subunit of each complex contains the polymerase activity and the 3′-exonuclease activity (except for Pol α); the Pri1 subunit of the Pol α -primase complex has the primase activity. Proposed replication functions are shown. For details, see Ref. 3.
|
Genes and subunit sizes
|
Activity
|
Fidelity
|
Function
|
|||
|---|---|---|---|---|---|---|
| S. cerevisiae | S. pombe | Human | ||||
| Pol α-primase | POL1-p167 | pol1-p159 | POLA1-p166 | Polymerase | 10-4-10-5 | Initiation of replication |
| POL12-p79 | pol12-p64 | POLA2-p68 | Initiation of Okazaki fragments | |||
| PRI1-p48 | pri1-p52 | PRIM1-p48 | Primase | |||
| PRI2-p62 | spp2-p53 | PRIM2A-p58 | ||||
| Pol δ | POL3-p125 | pol3-p124 | POLD1-p124 | Polymerase | 10-6-10-7 | Elongation and maturation of Okazaki fragments |
| POL31-p55 | cdc1-p51 | POLD2-p51 | 3′-Exonuclease | |||
| POL32-p40 | cdc27-p42 | POLD3-p66 | ||||
| cdm1-p19 | POLD4-p12 | |||||
| Pol ε | POL2-p256 | pol2-p253 | POLE-p261 | Polymerase | 10-6-10-7 | Replisome assembly |
| DPB2-p78 | dpb2-p67 | POLE2-p59 | 3′-Exonuclease | Leading strand synthesis | ||
| DPB3-p23 | dpb3-p22 | POLE3-p17 | DNA binding | |||
| DPB4-p22 | dpb4-p24 | POLE4-p12 | ||||