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. Author manuscript; available in PMC: 2009 Feb 12.
Published in final edited form as: J Steroid Biochem Mol Biol. 2007 May 16;105(1-5):106–114. doi: 10.1016/j.jsbmb.2006.12.104

Fig. 6.

Fig. 6

COBRA assay of ERα gene promoter C in wild-type and LTED cells. First PCR products of bisulfite converted DNA from wild-type or LTED cells using primers ERPROCBSF and ERPROCBSR were digested with restriction enzyme Hpy188III as described in Section 2. Rectangle indicates the position of the CpG site to be tested. Underline indicates the recognition site of Hpy188III that will be generated by bisulfite conversion of methylated DNA, but not unmethylated one. An image of the polyacrylamide gel electrophoresis is shown below. U, untreated. D, digested with Hpy188III.