Retina sections (14 μm thickness, dorsal of the optic nerve) of P20 mice were stained with antibodies against S-opsin (A–C) and M/L-opsin (D–F) with and without 11-cis retinal injections: (A, D) C57BL/6 wt mice; (B, E) Lrat−/− mice; (C, F) Lrat−/− mice with11-cis injections. For the untreated knockout mice, the opsin was distributed throughout the entire cell (cone inner and outer segment, cell body, axon and synaptic pedicle). Upon 11-cis retinal administration, less cone opsin was localized inappropriately to the synaptic pedicle, whereas more cone opsin was appropriately localized to the outer segment. Abbreviations: OS, outer segment; IS, inner segment; CB, cell body; Ax, axon; SP, synaptic pedicle. Scale bar = 25 μm.