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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Immunol. 2008 Jun 1;180(11):7537–7545. doi: 10.4049/jimmunol.180.11.7537

FIGURE 1.

FIGURE 1

L. braziliensis axenic amastigotes are highly efficient in activating DCs. Bone marrow-derived DCs (BMDCs) derived from B6 mice were infected with promastigotes (Pm) and amastigotes (Am) of L. braziliensis or L. amazonensis at the indicated parasite-to-cell ratios for 24 h. A, CFSE-labeled parasites were used to infect DCs and measure the percentage of infected cells (gated on CD11c and CFSE). B, IL-12p40 production in the culture supernatants of Pm- or Am-infected DC was assayed by ELISA. Dotted line represents baseline production by uninfected DCs (1.40 ± 0.1 ng/ml). Shown are representative results from three independent repeats. Bars represent statistically significant differences between the two Leishmania species. *, p < 0.05 and **, p < 0.01 indicate statistically significant differences between the control and infected groups by two-way ANOVA analysis.