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. 2009 Feb 24;4(2):e4559. doi: 10.1371/journal.pone.0004559

Figure 1. Western blot analysis of (A) Gαi2 and (B) Gβ2 proteins in shRNA, siRNA and ASO treated RAW 264.7 cells.

Figure 1

Two independent experiments are shown for the siRNA and ASO treatments. Blots from the shRNA-expressing lentiviral (LV) lines are representative of multiple samples taken over a 5-week period. Significant target gene knockdown is observed with all three platforms. UGIP: control lentivirus transfected cell line, UT: untreated, Mock: mock-treated. (C) Schematic diagram of the lentiviral construct used to generate shRNA expressing RAW 264.7 cell lines. The hairpin form of siRNA is expressed under the control of a mouse H1RNA polymerase III promoter. The vector also contains the enhanced GFP marker gene and the puromycin resistance gene (Puro) regulated by a UbiC promoter. IRES, internal ribosome entry site; FLAP, HIV-1 FLAP element; WRE, woodchuck hepatitis post- transcriptional regulatory element.