Figure 2. Hierarchically clustered dendrograms of gene expression changes.

Clustering was achieved using the CLUSTER and TREEVIEW programs (http://rana.lbl.gov/EisenSoftware.htm). Each row represents a gene, and each column represents a particular sample. (A) Gαi2 gene knockdown (LV40 and LV11: shRNA- expressing lentivirus transfected cell lines; I and L: I or L form of siRNA transfected into cells). (B) Gβ2 gene knockdown (LV4 and LV43: shRNA-expressing lentivirus transfected cell lines; G and H: G or H form of siRNA transfected into cells; Gb2-ASO: antisense oligonucleotide). The expression level relative to that of the control cells is provided by colors shown in log₂ scale. The target genes, Gαi2 and Gβ2, are highlighted by arrows. The apparent down-regulation of IL13ra2 in the Gαi2-deficient cells and IL1rn in the Gβ2-deficient cells are also highlighted (see context).