Table 3. Ligand-induced gene expression changes in Gαi2-deficient cells.

Gene	Assay	W vs Gi2-sh	LPS 0.5 h	Gi2: LPS 0.5 h	F: LPS	Pam2 0.5 h	Gi2: Pam2 0.5 h	F: Pam2	PGE 0.5 h	Gi2: PGE 0.5 h	F: PGE
Nfkbiz	Microarray	1.03	24.94	30.70	5.76	27.80	30.05	2.25	1.41	1.34	−0.07
	QRT-PCR		72.78	123.26	50.48	86.74	131.52	44.79	1.48	1.34	−0.14
Egr2	Microarray	0.60	12.11	18.10	5.99	14.80	20.74	5.94	0.71	0.78	0.14
	QRT-PCR		21.17	25.23	4.06	16.86	45.33	28.47	0.76	0.52	−0.62
1810011O10Rik	Microarray	1.01	4.81	10.16	5.35	4.00	8.63	4.63	1.42	1.34	−0.09
	QRT-PCR		53.51	84.18	30.67	71.06	116.53	45.47	3.45	2.32	−1.13
Tnf	Microarray	0.92	7.99	13.17	5.18	7.93	18.22	10.29	0.69	0.66	−0.06
	QRT-PCR		15.44	23.70	8.26	15.86	20.93	5.07	0.92	0.55	−0.73

Note: The numbers are in fold differences. F (F factor) was calculated by (ligand effect in knockdown) – (ligand effect in wild type): Ligand effect = 2^X−1, if X≥0 or  = 1−1/2^X, if X<0.

Where, X is the log₂ ratio.