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. Author manuscript; available in PMC: 2009 Feb 12.
Published in final edited form as: Adv Exp Med Biol. 2008;630:19–34. doi: 10.1007/978-0-387-78818-0_2

Figure 2.

Figure 2

A–C) Wild–type MCF-7 and LTED cells, deprived of E2, were treated with different concentrations of E2. Cytosols were measured for PgR (A), pS2 protein (B) and ERE-TK-CAT activity (C) 48 h after E2 treatment. A–C) From: Yue W et al. Endocrinology 2002; 143(9):3221-9;9 with permission of The Endocrine Society. D–F) ER trans-activation function in wild-type MCF-7 and LTED cells under basal conditions. Wild type and LTED cells were deprived of estrogen and transfected with ERE-TK-CAT (D), pERE-2-TK-CAT E) or pERE-E1b-CAT (F) reporter plasmids in conjunction with pCMV-beta Gal plasmid as internal control. Two days later, cell cytosols were collected and assayed for CAT activities using the same amount of beta-galactosidase units.9,11,13 D–F) From: Jeng MH et al. Endocrinology 1998; 139(10):4164–74;13 with permission of The Endocrine Society.