Table 1. Fluorescence response properties of VSFP2.1 variants.
ΔF/F of the donor at emission peak | ΔF/F of the acceptor at emission peak | ΔR/R | Number of cells | |
VSFP2.3 | −8.3±1.6% (470 nm) | 3.9±1.3% (520 nm) | 13.3±3.4% | 5 |
VSFP2.4 | −9.4±0.6% (520 nm) | 1.9±0.3% (613 nm) | 12.4±1.0% | 5 |
Mermaid | −6.8±2.7% (500 nm) | 5.1±1.4% (558 nm) | 12.9±4.8% | 5 |