Figure 4.

Single-channel properties of FLAG-tagged ENaC channels. (Upper) A single-channel recording performed in cell attached configuration with Na⁺ ions as major cation in the pipette. Pipette voltage (V_pip) was 80 mV. Downward deflections represent channel openings. The four distinct current levels indicate the presence of at least four conducting channels in the patch. (Lower) Current-voltage relationship in the presence of Na⁺ or Li⁺ ions in the pipette. Single-channel conductance estimated from regression analysis between −50 mV and −150 mV was 5.5 pS for Na⁺ and 8.8 pS for Li⁺.