Table 1.

Clinical profiles of patients and tumors, and summary of the presence of RNA, recombinant HLA-B7, and increase in CD3⁺ cell infiltration after gene transfer

Patient no.	Patient I.D.	Age/ Sex	HLA haplotype	Previous treatment	Site	DNA dose, μg (group)	RNA*	HLA-B7†	CD3+ infil- tration
1	G.M.	50/M	A2, 19; B35−, w6; C4,−; DR1, 12, 52; DQ1, 3, 7	BCG; chemotherapy; IL-2; TIL; limb perfusion with melphalan, tumor necrosis factor, and interferonγ	Left thigh	3(I), 30(II), 100(III)	+	++	+++
2	A.P.	46/F	A1, 3; B8, 27, w6, w4; C2, 7; DR13, 15	Surgery, radiation, chemotherapy, activated primed T cells, and IL-2	Right lateral thigh, right gluteal, right shoulder	3(I), 30(II)	+	ind.	+++
3	A.H.	54/M	A1, 2; B8, w6; C7; DR1, 15; DQ1, 6, 51	Surgery, chemotherapy, and IL-2	Left chest	3	−	+	++
4	I.M.	72/F	A2, 29; B8, −, w6; DR3, −, 52	Surgery	Left knee	30	+	+	ND
5	G.L.	40/F	A11, 30; B13, 44, w4, w6; C3, 6; DR2, 7, 53; DQ1, 2, 5, 51	Surgery, and limb perfusion with tumor necrosis factor and interferon α	Right thigh	100	+	ind.	−
6	D.M.	52/F	A2, −; B13, −, w4; C6, −; DR7, 8, 53; DQ2, 4	Surgery and interferon	Scalp	100	+	+/−	ND
7	F.B.	68/F	A23, 24; B13, 44, w4, w6; C4, −; DR7, −, 53; DQ2, 3	Surgery and radiation	Left back	100	+	++	+
8	M.S.	45/F	A3, 29; B44, 60; DR7, 13, 52, 53; DQ1, 2	Surgery	Right thigh	300	ND	+/−	++
9	G.B.	29/M	A3, 26; B18, 62, w6; C3, 5; DQ2; DR52, 53	Surgery and radiation	Right neck	300	+	+/−	++
10	J.C.	34/M	A1, 11; B44, −, w4; C5, −; DR1, 7, 53	Surgery, cisplatinum therapy, radiation, Dartmouth regimen, and chemotherapy	Left chest wall	300	−	ND	ND

I.D., Identification; M, male; F, female; BCG, Bacille Calmette–Guérin; ND, not determined; ind., indeterminate. 

^*RNA was detected by reverse transcriptase–PCR. 

^†HLA-B7 was detected by immunostaining.