Figure 1.

Alignment of the amino acid sequences (in single-letter code) predicted from a portion of the E1 and E2 genes of HCV, strain H77, spanning map positions 317–508 (13). H77 denotes the sample obtained from a plasmapheresis unit collected from patient H during the acute phase of posttransfusion NANB hepatitis (31), which represents the standard viral stock used for all the inocula, which had been previously titrated in chimpanzees (32). H77-1 and H77-2 denote two distinct sequences obtained independently in our laboratory by direct sequencing, following PCR amplification of cDNA from the same sample (H77). One of the two sequences was previously reported (23). The HVR1 of the E2 protein, spanning map position 384–414 (13), is shown in boldface type. The boxed sequences denote the sequence of the peptides (HVR1-A and HVR1-B) used for generating hyperimmune rabbit sera. Matches are indicated by vertical bars and changes are indicated by colons.