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Conformational analysis of plasmid released from scaffolds
| Lane | Sample | Nicked (%) | Linear (%) | Supercoiled (%) |
|---|---|---|---|---|
| 1 | molecular weight marker | -- | -- | -- |
| 2 | unincorporated DNA | 14.7 | 0.0 | 85.3 |
| 3 | DNA released between 8–24 hrs | 36.3 | 10.3 | 53.5 |
| 4 | DNA released between 1–3 days | 38.2 | 14.4 | 47.4 |
| 5 | DNA released between 3–7 days | 45.8 | 16.1 | 38.2 |
| 6 | DNA released between 7–14 days | 53.9 | 16.9 | 29.2 |
| 7 | DNA released between 14–21 days | 61.8 | 17.1 | 21.1 |
