The rise in endogenous synapsin I
phosphorylation due to 3×4 s-HFS is rapid, persists for at least 30
min, and is fully blocked by 100 μM APV or 10 μM KN-62.
Back-phosphorylation assays were performed on slices 30 sec
(n = 48 slice pairs, 8 rats) and 30 min
(n = 108 slice pairs, 18 rats) after receiving 3×4
stimulation in aCSF, and 30 min after receiving 3×4 stimulation in 100
μM APV (n = 54 slice pairs, 9 rats) or 10 μM
KN-62 (n = 16 slice pairs, 4 rats). Each bar
represents the mean back-phosphorylation as % of control (±SEM).
asterisks indicate significant differences from control slices
(P < 0.05, two-tailed t test).