Figure 2. ER stress induces Akt activity in a PERK-dependent manner.
(A) NIH-3T3 cells or (B) wild type and PERK−/− fibroblasts were treated with 2µg/mL tunicamycin or DMSO as a vehicle control for the indicated intervals. Cell lysates were resolved by SDS-PAGE and membranes were probed with antibodies for phosphorylated and total Akt and eIF2α.