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. Author manuscript; available in PMC: 2010 Jan 13.
Published in final edited form as: Biochemistry. 2009 Jan 13;48(1):140–147. doi: 10.1021/bi801659e

Figure 4.

Figure 4

Time dependence of cob(II)alamin concentration following pulsed laser photolysis of AdoCbl, and overlaid best-fit functions (red). Cob(II)alamin concentration was obtained from the absorbance at 470 nm. (a) Anaerobic solution; second-order plus constant fit function. (b) In EAL in aerobic solution; biexponential plus constant fit function. (c) In EAL in aerobic solution, with (S)-1-amino-2-propanol bound; monoexponential plus constant fit function The decay has been multiplied by a factor of 2.0 to account for the two-fold higher concentration AdoCbl in (b) relative to (c). The fitting parameters are presented in Table 2.