Table 1.
Stable Cell Line | Colonies per Plate |
|||||
---|---|---|---|---|---|---|
Experiment 1 |
Experiment 2 |
Experiment 3 |
||||
Control | Adriamycin | Control | Adriamycin | Control | Adriamycin | |
Vector | 3,460 | 303 | 2,550 | 162 | 3,090 | 379 |
nSMase3 55 | 2,930 | 30 | 2,590 | 11 | 3,080 | 78 |
nSMase3 61 | 3,550 | 180 | 2,790 | 104 | 3,640 | 280 |
NOTE: RKO human colon cancer cells stably expressing pcDNA3.1-Myc or pcDNA3.1-Myc-nSMase3 were treated with 20 nmol/L Adriamycin for 24 h. Cells were then cultured in medium without Adriamycin for 10 to 14 d and the numbers of colonies were counted.