Skip to main content
. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Mol Cancer Res. 2008 May;6(5):795–807. doi: 10.1158/1541-7786.MCR-07-2097

Table 1.

nSMase3 Overexpression Sensitizes RKO Cells to Adriamycin-Induced Apoptosis

Stable Cell Line Colonies per Plate
Experiment 1
 Experiment 2
 Experiment 3
Control Adriamycin Control Adriamycin Control Adriamycin
Vector 3,460 303 2,550 162 3,090 379
nSMase3 55 2,930 30 2,590 11 3,080 78
nSMase3 61 3,550 180 2,790 104 3,640 280

NOTE: RKO human colon cancer cells stably expressing pcDNA3.1-Myc or pcDNA3.1-Myc-nSMase3 were treated with 20 nmol/L Adriamycin for 24 h. Cells were then cultured in medium without Adriamycin for 10 to 14 d and the numbers of colonies were counted.