Figure 2.

Ankyrin-B protein levels decrease in the border zone 5 days post-occlusion. (A) Representative immunoblots and (B) densitometric measurements (normalized to actin and expressed relative to normal levels) of AnkB from remote and BZ regions of normal and infarcted hearts. Error bars designate standard deviation (*P < 0.01 compared with normal BZ or remote, n = 4 for normal, n = 5 for 5-day, and n = 3 for 14+ day infarct). Densitometric measurements were made from all samples analysed on same gel and normalized to the corresponding actin levels from the same blot. Equal protein loading was ensured by BCA assay and verified by analysis of Coomassie and Ponceau stains of gel and blots.