Figure 5.

Effect of siRNA knockdown of CYP1A1 and CYP1B1 gene expressions. Human umbilical vein endothelial cells were treated with either CYP1A1 (A) or CYP1B1 (B) siRNA 48 h prior to 24 h of 25 dynes/cm² shear stress (n = 4). mRNA levels of CYP1A1, CYP1B1, and thrombospondin-1 were compared with cells treated with a non-silencing nonsense control and subjected to identical shear conditions. The knockdown of CYP1A1 caused significant decreases in CYP1B1 and thrombospondin-1. The knockdown of CYP1B1 caused no significant gene changes other than the target knockdown of CYP1B1. Significance was measured using paired Student's t-test; *P < 0.05 between targeted knockdown and nonsense control.