Table 2.
Motility analysis of wild type and Apoer2 mutant sperm
WT | Apoer2[ex19] | Apoer2[Δex19] | Apoer2[Stop] | Apoer2[Dab] | Apoer2 KO | |
---|---|---|---|---|---|---|
% Motile | 69.0 ± 5.6 | 46.3 ± 10.3 | 30 ± 7.1 | 48.3 ± 7.5 | 32.7 ± 9.4 | 18.7 ± 7.3 * |
| ||||||
Curvilinear velocity, μm/s | 304.6 ± 4.0 | 283.1 ± 11.2 | 233.0 ± 2.7 * | 242.6 ± 7.4 * | 145.3 ± 7.1 * | 160.4 ± 25.0 * |
Average path velocity, μm/s | 140.0 ± 0.4 | 142.1 ± 2.3 | 114.2 ± 3.6 * | 116.4 ± 2.8 * | 85.0 ± 6.2 * | 80.1 ± 9.9 * |
Straight line velocity, μm/s | 103.2 ± 2.6 | 116.6 ± 1.6 | 87.1 ± 5.2 | 87.9 ± 5.0 | 66.9 ± 7.8 | 54.8 ± 7.5 * |
Amplitude of lateral head displacement, μm | 16.6 ± 0.2 | 14.0 ± 0.6 | 12.3 ± 0.2 * | 12.8 ± 0.2 * | 7.7 ± 0.4 * | 9.2 ± 1.6 |
Beat cross frequency, Hz | 24.7 ± 1.4 | 26.3 ± 1.1 | 28.2 ± 0.3 | 28.5 ± 0.7 | 30.2 ± 1.6 | 30.5 ± 1.3 |
Linearity, % | 34.6 ± 0.6 | 42.6 ± 1.8 | 39.1 ± 1.8 | 37.1 ± 2.0 | 48.0 ± 4.9 | 37.5 ± 0.8 |
Spermatozoa, 150 from each of three animals per genotype, were analyzed for percent motility and various motility parameters, (mean ± S.E.)
P<0.01