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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Mol Endocrinol. 2008 Oct 24;42(1):19–33. doi: 10.1677/JME-08-0088

Figure 4.

Figure 4

Effects of (A) RNA interference or (B) ICI 182780 on E2-dependent induction or repression of genes. MCF-7 cells were transfected with iNS or iSP and treated with DMSO or 20 nM E2 or treated with DMSO, E2 alone, or E2 plus 2 μM ICI 182780, and gene expression was determined by real-time PCR as described in the Materials and methods. Results are means±s.e.m. for three replicate determinations and significant (P<0·05) induction or repression by E2 (*) and reversal of these effects by iSP or ICI 182780 (**) is indicated.