FCS autocorrelation data and corresponding best fits of free AEE QDs (top, left), vesicles labeled with the dye lipid TRITC-DHPE (top, right), maleimide-functionalized AEE QDs bound to DHPTE-containing vesicles (bottom, left), and DHPTE-containing vesicles with bound and unbound maleimide-functionalized AEE QDs (bottom, right). The data illustrate that QD-labeled vesicles can be prepared without free, excess QDs.