Fig. 5.

[Ca²⁺] dynamics in glomeruli: role of CNG channels. (A) Kinetic changes in fluorescence (ΔF/F₀) in axon termini of OSN in hemi-head preparation challenged with an odor puff applied locally under control conditions. (B) Same glomerulus as in A, but challenged with an odor puff in the presence of the AC blocker, SQ22536. In C and D the pseudocolor images showing the images of the same glomerulus the fluorescence kinetic changes of which are shown in A and B. Images in C refer to the glomerulus in control conditions and those in D to the same glomerulus treated with the AC inhibitor. After the first stimulation the hemi-head was extensively washed with fresh medium and the second challenge with the odors in the presence of the AC inhibitor carried out 15 minutes after the first. (E) Summary of response (%) (mice, n = 4; glomeruli, n = 4; P = 0.01**; bars, SEM). ACSF was supplemented with TTX (4 μM) in all experiments. White circle indicates responding glomerulus.