Fig. 3.

Oxidation increases formation of inter-subunit disulfide bonds. (A and B) Effect of H₂O₂ on ASIC1a Ads2 formation at 37 °C (A) or 4 °C (B). ASIC1a-transfected cells were treated with different concentrations of H₂O₂ for 15 min. After the indicated treatments, surface protein was biotinylated, isolated by NeutrAvidin pull-down, and analyzed under non-reducing conditions. (C) Quantification of ratio of Ads2 to total (estimated as monomer plus Ads2 plus AdsH). (D) Quantification of ratio of AdsH to total.