Table 6. Positive selection on single codon level based on PAML (see text for details).
Genea | Modelsb | ωSc | p(ωS)d | −2ΔLLb | Pb | aae |
GNBP | M1 vs. M2 | 1 | 9.1 | 0 | Ns | None |
GNBP | M7 vs. M8 | 1 | 3.8 | 5.9 | Ns | None |
SP14D1 | M1 vs. M2 | 1 | 1.1 | 0 | Ns | None |
SP14D1 | M7 vs. M8 | 1.97 | 2.4 | 6.6 | 0.037 | 206ns; 169ns |
Gambicin | M1 vs. M2 | 12.1 | 1.3 | 6.4 | 0.041 | 72** |
Gambicin | M7 vs. M8 | 11.1 | 1.3 | 11.8 | 0.001 | 72** |
Defensin | M1 vs. M2 | 1.4 | 0 | 1.1 | Ns | None |
Defensin | M7 vs. M8 | 2.2 | 2.4 | 0.9 | Ns | None |
GNBP alignment was 171 aa long and included eight species; SP14D1alignment was 246 aa long and included six species; Gambicin alignment was 81 aa long and included nine species; Defensin alignment was 101 aa long and included seven species (see Materials and Methods for the species listing for each gene).
Likelihood ratio tests (with 2 df) were used to determine the significance of finding ω>1 over all codons by comparing selection models (M2 and M8) that allowed for ω>1 with neutral (M1 and M7) models that allowed only ω≤1.
Estimate of the highest ω value for any codon.
The proportion of codons with the highest w estimate.
Positions of the amino acids with ω>1 and their significant value estimated by BEB test in PAML.