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. 2009 Feb 27;5(2):e1000316. doi: 10.1371/journal.ppat.1000316

Figure 4. Interpain A inhibits the lectin pathway.

Figure 4

Mannan was immobilized on microtiter plates and allowed to activate 4% NHS (for MBL, C4b, C3b assays) and 10% NHS (for C9 assay) that was pre-incubated for 15 min with various concentrations of InpA and InpAC154A. After 20 min (C3b, C4b) and 45 min (C9, MBL) of incubation, the plates were washed, and deposited MBL (A), C4b (B), C3b (C), and C9 (D) were detected with specific polyclonal antibodies. Absorbance obtained in the absence of interpain was set as 1. An average of three independent experiments is presented with bars indicating SD.