Table 1.
S. cerevisiae strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| AFS34a | a ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15, trp1-1 | Straight et al. (1996) |
| AFS35a | α ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15, trp1-1 | A. Straight |
| AFS92 | As AFS34 except bar1Δ | Jaspersen et al. (1998) |
| BY4741b | a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Brachmann et al. (1998) |
| BY4742b | α his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Brachmann et al. (1998) |
| YEF473Ab | a his3-Δ200 leu2-Δ1 lys2-801 trp1-Δ63 ura3-52 | Bi and Pringle (1996) |
| YEF473Bb | α his3-Δ200 leu2-Δ1 lys2-801 trp1-Δ63 ura3-52 | Bi and Pringle (1996) |
| YEF1681 | as YEF473A except MYO1-GFP:kanMX6 | Bi et al. (1998) |
| yBRT135-1a | As AFS92 except pds1Δ::LEU2 clb5Δ::HIS3 trp1Δ::SIC1-10X:TRP1 | Thornton and Toczyski (2003) |
| yTMN19 | As AFS35 except apc2Δ::CAN1 pds1Δ::LEU2 clb5Δ::HIS3 trp1Δ::SIC1-10X:TRP1 | Thornton and Toczyski (2003) |
| GT049 | As AFS92 except MYO1-EGFP:His3MX6 | This study |
| GT050 | As AFS92 except IQG1-EGFP:His3MX6 | This study |
| GT052 | As AFS92 except MYO1-EGFP:His3MX6 cdh1Δ::LEU2 | This study |
| GT053 | As AFS92 except IQG1-EGFP:His3MX6 cdh1Δ::LEU2 | This study |
| GT073 | As yTMN19 except MYO1-GFP:URA3 | This study |
| GT074 | As yBRT135-1a except MYO1-GFP:URA3 | This study |
| GT079 | As AFS92 except MYO1-EGFP:His3MX6 cdh1Δ::LEU2 ase1Δ::URA3 | This study |
| GT081 | As AFS92 except MYO1-GFP:His3MX6 cdh1Δ::LEU2 [GAL-SIC1, URA3] | This study |
| GT104 | As AFS92 except MLC2-GFP:His3MX6 | This study |
| GT108 | As AFS92 except MYO1-GFP:His3MX6 [pCDC5-CDC5Δ5-70-myc9, URA3] | This study |
| GT110 | As AFS92 except MLC2-GFP:His3MX6 cdh1Δ::LEU2 | This study |
| GT124 | As BY4741 except IQG1Δ42-TAP:His3MX6:LEU2 | Ko et al. (2007) |
| GT132 | As AFS92 except MYO1-Cherry:kanMX6 MLC2-GFP:His3MX6 cdh1Δ::LEU2 | This study |
| GT133 | As BY4741 except MYO1-Cherry:kanMX6 IQG1-GFP:His3MX6 cdh1Δ::LEU2 | This study |
| GT134 | As BY4741 except MYO1-Cherry:kanMX6 SEC2-GFP:His3MX6 cdh1Δ::LEU2 | This study |
| GT158 | As YEF473A except MYO1-GFP:kanMX6 mlc2Δ::His3MX6 | This study |
| GT160 | As YEF473A except MYO1-GFP:kanMX6 iqg1Δ::His3MX6 | This study |
| GT165 | As BY4741 except cdh1Δ::LEU2 | This study |
| GT166 | As BY4742 except cdh1Δ::LEU2 | This study |
| GT178c | MYO1-GFP:kanMX6 iqg1Δ::His3MX6 cdh1Δ::LEU2 | GT160 × GT166 |
| GT200 | As BY4742 except MYO1-GFP:His3MX6 cdh1Δ::LEU2 | This study |
| GT203c | MYO1-GFP:kanMX6 mlc2Δ::His3MX6 cdh1Δ::LEU2 | GT158 ×GT165 |
| GT204 | As BY4741 except MYO1-GFP:His3MX6 cdh1Δ::LEU2 spo12Δ::kanMX6 | This study |
| GT208 | As AFS92 except [MET25p-yEGFP3-MLC1, HIS3, CEN/ARSH4] | This study |
| GT209 | As AFS92 except [MET25p-yEGFP3-MLC1, HIS3, CEN/ARSH4] cdh1Δ::LEU2 | This study |
| GT225 | As AFS92 except MYO1-CHERRY:kanMX6 cdh1Δ::LEU2 MET25p-yEGFP3-MLC1, HIS3, CEN6/ARSH4 | This study |
| GT229 | As BY4741 except MYO1-GFP:His3MX6 cdh1Δ::LEU2 clb2Δ::kanMX6 | This study |
| GT235 | As AFS92 except MYO1-GFP:His3MX6 cdh1Δ::LEU2 hsl1Δ::TRP1 swe1Δ::LEU2 | This study |
| GT262 | As BY4741 except MYO1-GFP:His3MX6 cdh1Δ::LEU2 fin1Δ::kanMX6 | This study |
| KO255 | As YEF473A except cdh1Δ::TRP1 | Ko et al. (2007) |
| Myo1-GFP | As BY4741 except MYO1-GFP:His3MX6 | Huh et al. (2003) |
| RNY2349 | As YEF473A except MYO1-GFP:kanMX6 cdh1Δ::TRP1 | This study |
| RNY2356 | As BY4741 except MYO1-GFP:His3MX6 mlc2Δ::kanMX6 | This study |
| RNY2369 | As BY4741 except MYO1-GFP:His3MX6 cdh1Δ::LEU2 | This study |
| RNY2371 | As BY4741 except MYO1-GFP:His3MX6 mlc2Δ::kanMX6 cdh1Δ::LEU2 | This study |
| RNY2373 | As BY4741 except MYO1-GFP:His3MX6 IQG1Δ42-TAP:His3MX6:LEU2 | This study |
| RNY2375 | As BY4741 except MYO1-GFP:His3MX6 mlc2Δ::kanMX6 IQG1Δ42-TAP:His3MX6:LEU2 | This study |
a W303 strain background.
b S288C strain background. Two different S288C strain families were used, derived from strains BY4741/BY4742 or YEF473.
c These strains have a hybrid S288C background resulting from crossing strains of the BY4741/BY4742 and YEF473 backgrounds, as indicated in the Source column.